Genetic and Genomic Sequencing

Dr. Richard Myers and his group at the HudsonAlpha Institute oversee state of the art efforts in genetics and genomics, including genome-wide methylation assays, RNASeq, and Deep Sequencing capabilities. The Institute has six Illumina Genome Analyzer IIx sequencers (right), four Illumina HiSeq machines, one ABI SOLiD and one Roche 454 sequencer. The servers currently include more than 700 TB of usable storage space and more than 250 Intel Nehalem processors dedicated to sequencing and storage analysis.

Genotyping Platform

Genotyping of human genomic DNA is performed at Cornell University using Taqman 5’ exonuclease real-time PCR assays. Thermal cycling and fluorimetric quantitation of amplification is performed on an Applied Biosystems 7900HT apparatus.