TRANSCRIPT PROFILING SHOWS SELECTIVITY OF ANATOMICAL REGIONS AND PHYSIOLOGICAL PATHWAYS AFFECTED IN THE BRAINS OF PATIENTS WITH MOOD DISORDERS
P.V. Choudary; S.J. Evans; M.P. Vawter; H. Tomita; J. Li; J. Meador-Woodruff; J.F. Lopez;. R.M. Myers; W.E. Bunney; S.J. Watson; H. Akil; E.G. Jones
Society for Neuroscience 32nd annual meeting. 2002.
We performed global gene expression profiling of 9 individuals diagnosed with Bi-Polar illness and 11 with Major Depressive Disorder (MDD) using Affymetrix GeneChips (HG_U95Av2). Total RNA was isolated separately from anterior cingulate cortex (AnCg), dorsolateral prefrontal cortex (DLPFC) and cerebellum (CB) of postmortem brains from patients and individually-matched controls. Each RNA preparation was split into 2 identical samples and processed independently as chip duplicates, with sample labeling, hybridization and scanning performed at two different sites. Probe sets that showed statistically significant (p<0.05) changes (increase/decrease) in each region were identified and clustered using various combinations of statistical analytical programs, Microarray Suite 5.0, dCHIP and GeneSpring. Abstracts by Tomita et al. and Evans et al. describe signaling pathways affected in Bi-Polar AnCg, and growth factor pathways altered in MDD DLPFC. Here, we describe how genes and functional pathways identified in these companion studies are affected uniquely in a single region, or are altered commonly across cortex and cerebellum, and whether these pathways converge/ diverge between Bi-Polar illness and Major Depressive Disorder. Supported by: The Pritzker Neuropsychiatric Disorders Research Consortium, Pritzker Family Philanthropic Fund and NIH CONTE Center grant #L99MH60398.