Patterns of Differential Gene Expression In Schizophrenia Overlap In Cortical Regions
Marquis P. Vawter; Simon Evans; Prabha Choudary; Mary Atz; Hiroaki Tomita; Ben Bolstad; Jun Li; Terry Speed; Rick Myers; Stanley J. Watson; E.G. Jones; Huda Akil; William E. Bunney
American Society of Human Genetics 53nd annual meeting . 2003.
This project involves a multi-site collaborative effort to investigate gene expression in neuropsychiatric disorders in multiple brain regions. We have investigated schizophrenia (n = 9) and controls (n = 9) by microarray analysis using Affymetrix U133A and B chips. We report on the microarray comparison of three cortical regions: dorsolateral prefrontal cortex (DLPFC), anterior cingulate, and entorhinal cortex (ERC). We selected the top and bottom differentially expressed genes for schizophrenia from a robust probe level linear model analysis (http://stat-www.berkeley.edu/users/bolstad/AffyExtensions/AffyExtensions.html) for each brain region. The top 500 and bottom 500 changed genes were further classified using Gene Ontology and KEGG classifications. There was over-representation of ribosomal genes, heat shock related genes, chaperone genes, and immune genes in cortical regions. The preliminary gene classifications for the ERC, DLPFC and anterior cingulate will be compared to the results from the hippocampus. The preliminary microarray evidence suggests several cortical regions share overlapping expression patterns for genes dysregulated in schizophrenia. This work was supported by NIH Conte Center Grant MH60398-03 and the Pritzker Neuropsychiatric Disorder Research Consortium Fund.